Human Anti-PEG Recombinant Antibody (clone E11)
CAT#: FAMAB-1525CQ
This product is a recombinant human antibody that recognizes PEG. The antibody was purified by affinity chromatography. It can be used in WB, ELISA, FC.
Tested Data
SDS-PAGE
Figure 1 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SDS-PAGE
SDS-PAGE analysis of FAMAB-1525CQ in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
SEC-HPLC
Figure 2 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SEC-HPLC
The purity of FAMAB-1525CQ was greater than 95% as determined by SEC-HPLC.
Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl
DB
Figure 3 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in DB
Dot Blot analysis of FAMAB-1525CQ was performed by coating with PEG6000.
FAMAB-1525CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG Fc antibody
ELISA
Figure 4 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in ELISA
ELISA analysis of FAMAB-1525CQ was performed by coating with PEG6000. Then blocked with BSA and incubated with FAMAB-1525CQ. The HRP-conjugated goat anti-human IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
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Specifications
- Immunogen
- PEG-modified E. coli β-glucuronidase
- Host Species
- Human
- Type
- Human IgG1
- Specificity
- The repeating subunits of the PEG backbone
- Clone
- E11
- Applications
- WB, ELISA, FC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Polyethylene Glycol
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-1525CQ, RRID: AB_3111335)
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone E11"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2659 | Hi-Affi™ Recombinant Rabbit Anti-PEG Monoclonal Antibody (DS2659AB) | ELISA, WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0771LY-S(P) | Human Anti-PEG Recombinant Antibody; scFv Fragment (HPAB-0771LY-S(P)) | ELISA, FC, IHC | Chimeric scfv |
| HPAB-0772LY-S(P) | Human Anti-PEG Recombinant Antibody; scFv Fragment (HPAB-0772LY-S(P)) | ELISA, FC, IHC | Chimeric scfv |
| HPAB-0696-FY-F(E) | Mouse Anti-PEG Recombinant Antibody; Fab Fragment (HPAB-0696-FY-F(E)) | ELISA, FC | Mouse Fab |
| PABJ-0028-S(P) | Mouse Anti-PEG Recombinant Antibody (clone 3.3); scFv Fragment | ELISA | Mouse scFv |
| PABJ-0029-S(P) | Mouse Anti-PEG Recombinant Antibody (clone 2B5); scFv Fragment | ELISA | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0771LY | Human Anti-PEG Recombinant Antibody (HPAB-0771LY) | ELISA, FC, IHC | Chimeric (mouse/human) IgG1 |
| HPAB-0772LY | Human Anti-PEG Recombinant Antibody (HPAB-0772LY) | ELISA, FC, IHC | Chimeric (mouse/human) IgG1 |
| HPAB-0697-FY | Mouse Anti-PEG Recombinant Antibody (HPAB-0697-FY) | ELISA, FC, WB | Mouse IgG1 |
| HPAB-0698-FY | Mouse Anti-PEG Recombinant Antibody (HPAB-0698-FY) | ELISA, FC, WB | Mouse IgG1 |
| HPAB-0699-FY | Mouse Anti-PEG Recombinant Antibody (HPAB-0699-FY) | ELISA, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0771LY-F(E) | Human Anti-PEG Recombinant Antibody; Fab Fragment (HPAB-0771LY-F(E)) | ELISA, FC, IHC | Chimeric (mouse/human) Fab |
| HPAB-0772LY-F(E) | Human Anti-PEG Recombinant Antibody; Fab Fragment (HPAB-0772LY-F(E)) | ELISA, FC, IHC | Chimeric (mouse/human) Fab |
| HPAB-1179WJ-F(E) | Mouse Anti-PEG Recombinant Antibody (clone 157D 29G1); Fab Fragment | ELISA, WB | Mouse Fab |
| PABJ-0028-F(E) | Mouse Anti-PEG Recombinant Antibody (clone 3.3); Fab Fragment | ELISA | Mouse Fab |
| PABJ-0029-F(E) | Mouse Anti-PEG Recombinant Antibody (clone 2B5); Fab Fragment | ELISA | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0696-FY | Mouse Anti-PEG Recombinant Antibody (HPAB-0696-FY) | ELISA, FC, WB | Mouse IgM |
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