Human Anti-PEG Recombinant Antibody (clone E11) (CAT#: FAMAB-1525CQ)

Figure 1 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SDS-PAGE

SDS-PAGE analysis of FAMAB-1525CQ in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.

Figure 2 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in SEC-HPLC

The purity of FAMAB-1525CQ was greater than 95% as determined by SEC-HPLC.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in DB

Dot Blot analysis of FAMAB-1525CQ was performed by coating with PEG6000.

FAMAB-1525CQ incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Human IgG Fc antibody

Figure 4 Human Anti-PEG Recombinant Antibody (FAMAB-1525CQ) in ELISA

ELISA analysis of FAMAB-1525CQ was performed by coating with PEG6000. Then blocked with BSA and incubated with FAMAB-1525CQ. The HRP-conjugated goat anti-human IgG Fc as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.